Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells

Abstract Targeted liposomes are designed to target specific receptors overexpressed on the surfaces of cancer cells. This technique ensures site-specific drug delivery reduce undesirable side effects while enhancing efficiency encapsulated therapeutics. Upon reaching tumor site, these can be triggered release their content in a controlled manner using ultrasound (US). In this study, from pegylated calcein-loaded modified with transferrin (Tf) and US was evaluated. Low-frequency at 20-kHz three different power densities (6.2 mW/cm 2 , 9 10 ) found increase calcein release. addition, transferrin-conjugated (Tf-PEG liposomes) were more sonosensitive compared non-targeted (control) liposomes. Calcein uptake by HeLa cells significantly higher Tf-PEG control further enhanced following exposure low-frequency (at 35 kHz). These findings show that targeted have promising potential as safe effective platform.